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Common Prenatal Tests:
Amniocentesis

What is amniocentesis?

During amniocentesis, a sample of amniotic fluid (the fluid around the baby) is removed from your uterus and sent to a laboratory for evaluation. Amniocentesis is performed by inserting a thin needle through your abdomen into your uterus (womb) and withdrawing a small amount of fluid. Your body will make more fluid to replace the fluid that is taken out. The baby will not be hurt during the procedure. Some women feel mild cramping during or after the procedure. Your doctor may tell you to rest on the day of the test, but usually you can resume normal activity the next day.

 

Technical

Procedure Name Ultrasound, Amniocentesis

Synonyms Amniocentesis Ultrasound

Procedure Commonly Includes Locating appropriate puncture site for amniocentesis and subsequent performance of amniocentesis under direct visualization.

Indications Used in the last two trimesters of pregnancy to determine post maturation, deformities, Rh incompatibility, amniotic fluid bilirubin level, amniotic fluid L/S ratio, amniotic fluid-fetoprotein level, and genetic malformations

Contraindications Inadequate amount of fluid, anterior placenta, presence of fetal vertex or trunk in needle path

Patient Preparation The examination may be long, up to 1 hour including waiting time. An emergency examination or an unpredictably long preceding examination may result in additional delay. Note: Each Rh-negative unsensitized woman whose husband is not known to be Rh-negative should receive Rh immune globulin.

Special Instructions Patient is brought to Radiology and the procedure is performed by a radiologist or obstetrician with the aid of ultrasound. Patient is required to stay in the hospital for a few hours for precautionary measures.

Complications Pain, amnionitis (infection), abortion, hemorrhage, trauma to fetus, rupture of membranes, placental or subchorionic hematoma, Rh sensitization, premature labor, abruptio placenta, maternal death

Equipment Standard B-mode real time ultrasonic imager with 2-5 MHz transducer. Biopsy guide if desired; 20- or 22-gauge spinal needle.

Technique Under ultrasound guidance, an appropriate location is found to insert a small needle into the amniotic fluid. The skin is marked appropriately and prepared for placement of the needle using sterile technique. Needle is placed and amniotic fluid aspirated.

Data Acquired Images of area to be sampled

Specimen Amniotic fluid

Causes for Rejection Patient not able to tolerate exam

Turnaround Time A typed report will generally be issued within 36 hours. A preliminary verbal report generally can be given to the referring physician on request.

Normal Findings Successful aspiration of adequate amount of amniotic fluid

Limitations Amniotic fluid not obtainable due to placenta location or lack of suitable puncture site, little or no amniotic fluid available for amniocentesis

References:
Platt LO, Hill LM, DeVore GR, et al, "Amniocentesis: Current Concepts and Techniques,"The Principles and Practice of Ultrasonography in Obstetrics Gynecology, Sanders and James, eds, Norwalk, CT: Appleton-Century-Crofts, 1985.

 

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